FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773024
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.037.5352868
GTCTCGC13250.036.730021
GTCTTGC23400.033.835521
TCGCTCT14050.033.8164634
GTACGGG2202.2258973E-829.7154331
TCTCGCT17250.027.543262
GTGTTAC13150.027.066531
GTATTAC7800.027.0063321
CGCTCTG16500.026.613775
CTCGCTC16650.025.9416483
CTGTCGC20700.025.3861839
CTCGATC10600.025.1270058
GCGTAAC1751.2404195E-424.9043621
GTATTAA9100.024.7447191
GTACTAG4800.024.2125741
GTATAAC5450.023.990441
GTGTAAT11100.023.558181
AGTACCG2252.8030334E-522.396295
GTGTTAG19100.021.6772251
GGTATAC5700.021.6638811
Produced by FastQC (version 0.11.5)