FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822455
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.039.326168
GTCTTGC26850.030.5124591
TCGCTCT13350.029.1271654
GTCTCGC12650.028.0833321
CTGTCGC17600.026.1799249
GTGTAAG14900.024.8157231
GTATAAG9650.024.041731
CTCGCTC15050.023.93383
GTCTTAC11650.023.6483441
GTGTAAA18450.023.577511
GGTATAC7150.023.321951
GTGTAAC8750.023.2002741
TACACCG2502.4205092E-623.0428245
GTGTTAG21500.022.2560731
TCTCGCT15900.022.2012332
GTATATA15400.022.1268831
TAATACT10100.022.1017194
GTCTCAC32350.021.739051
GTATTAA8100.021.4817331
GTGCTAC10800.021.4817331
Produced by FastQC (version 0.11.5)