FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956620
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13500.040.2708971
TCTGTCG12350.040.2224548
TCGCTCT15500.034.844444
TCTCGCT16200.032.4591182
TAATACT9100.030.070884
CGCTCTG16450.029.7678155
CTCGCTC17300.028.313383
GTCTTGC27800.028.1606541
CTGTCGC18050.027.919959
GTCTCAC35000.024.8528961
TATACTG9500.023.4986045
GTATTAG10550.023.3609371
GTGTTAC12600.023.011941
GTCGTAC1650.002604631221.9659421
TTGTCGC2353.918545E-521.4448979
GTCTTAC11850.021.4098421
GGGTACG2755.224063E-621.0873051
GTATAGA9350.020.9322531
TTGCTCT38200.020.924914
GTGTAAA19400.020.924261
Produced by FastQC (version 0.11.5)