FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956620
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12700.036.582731
TCTGTCG13100.035.720148
GTCTTGC26600.031.3845631
TCGCTCT15100.030.9905684
GTATTAG10500.029.0375421
CGCTCTG15650.027.6017995
GTACCGG2903.9108272E-1027.5356031
GTCGTAC1100.00961376126.3977661
CTCGCTC15900.025.808983
GTATTAA10050.025.2814431
TCTCGCT17600.024.9514392
TAATACT8800.024.5432284
GTGTTAA12600.024.1979521
CTGTCGC19550.023.5679
GTACTAT7650.021.8256031
GTATAAG10400.021.6385541
GTGTAAG14900.021.4371131
TAGTACT7400.021.4034634
GTGTAAC9500.021.3960841
GTCTCAC34600.020.9808851
Produced by FastQC (version 0.11.5)