FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818895
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.040.0349968
GTCGTAC1105.2641117E-639.549381
GTCTTGC26450.035.6368061
CTCGATA1352.248983E-531.996788
GTCTCGC12950.031.3544641
CTGTCGC16850.026.4899169
TCGCTCT14800.026.2732164
TCTCGCT15200.025.589082
GCGTAAC1450.001226285125.0024831
GTATTAG10550.024.7417931
GTGTACG4450.024.4406281
GTGTTAG20950.024.226751
GTGTAAT11150.023.4103971
CGCTCTG15600.023.0795145
GTATTAT9700.022.4249081
TTGCTCT36450.022.3234944
GTATAGT8450.022.3099061
GATCTAT5850.022.3099061
CTTGCTC34950.022.0516513
GTGCTAC11250.021.9132861
Produced by FastQC (version 0.11.5)