FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123744
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13450.042.2851878
GTCTCGC13300.038.734311
TCGCTCT15150.035.174484
GTCTTGC29050.033.9689331
TCTCGCT17250.031.7359352
CTCGCTC18200.029.2878283
CTGTCGC20300.028.0169879
CGCTCTG19000.026.9099795
GTACCGA1409.952276E-425.91381
GTCTCAC36500.023.2585241
GTATTAC9750.022.3257351
GTGTTAC14350.022.2479461
GTATAGA9900.021.9874671
GTTTCAC50050.021.7319983
CTCGATC12600.021.7118348
GGTATAC6100.021.4107481
GTACTAT6800.021.3407781
CTTGCTC39200.021.1319053
GTTAGCC17100.021.0620543
GTATTAG13450.021.0393091
Produced by FastQC (version 0.11.5)