FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123744
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.039.799661
TCGCTCT15200.035.046674
GTCTTGC27950.032.243391
TCTGTCG15000.030.2343718
CTCGCTC16950.028.030633
CGCTCTG15900.027.1656275
TCTCGCT17800.026.6916622
GGCGTAC1801.4962423E-424.2258821
GTACTAG5850.023.6047041
CTGTCGC22000.023.232049
TTGCTCT40050.023.0073224
GTGTTAC15100.022.621521
GGTATAC7100.022.519831
TCTGTCA38600.022.3792558
GTACCGA1952.74473E-422.1504336
GTATAAG8450.021.502261
GTCTTAC11550.021.3942831
GTCTCAC35850.021.083611
GGAGTGC64050.021.017845
CTTGCTC38500.020.754913
Produced by FastQC (version 0.11.5)