FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402808
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14600.039.940928
GTCTCGC17650.033.304681
GTCTTGC31400.031.663281
CTGTCGC18700.031.5688069
TCTCGCT18300.030.6865862
TCGCTCT17300.029.1298264
GGTATAC7250.029.0285241
GTATTAG12900.027.5658471
CTCGCTC19600.027.1803593
GTATTAT9650.025.5691681
CGCTCTG19050.024.1863845
GTGTAAC9850.023.5764681
TTGCTCT45000.023.0375294
GTATAAT12300.023.0104161
GTATTAA10450.022.9172571
TATACTG10200.022.5858145
GTGTAGC15450.022.5464291
CTGTTGC37250.022.4190739
GTATAAG8750.022.3934351
GTATAGA10050.022.3851811
Produced by FastQC (version 0.11.5)