FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402808
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC32700.030.5147111
TCTGTCG16050.030.4966378
TCGCTCT17600.028.6305034
GTCTCGC15500.028.4012621
GTACTAC6250.024.6522961
GTACGGG3301.54796E-924.4566421
GTATTAG12250.023.9575271
TACCCCG3352.2937456E-923.6383425
GTCTAAG11350.023.271521
GTATTAT12050.023.1374051
GTATAAC6100.022.8529281
GTACTAT7100.022.7343441
CTCGCTC20900.022.3871023
GTCTTAC14800.022.3084241
TCTCGCT21050.021.8856142
GTCTCAC42900.021.7202361
GTGTAAG17850.021.3738731
GGCTAGA21300.021.3565061
CTGTCGC24150.021.1621259
GTATATA15050.020.9628371
Produced by FastQC (version 0.11.5)