FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150866
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13350.039.9057738
CTGTCGC16300.033.1252339
GTCTCGC14350.031.3488861
GTATTAG10000.031.1997261
TCGCTCT15800.030.0726764
GTCTTGC31800.028.74921
GTATTAC7000.026.949931
CTCGCTC17650.025.6977393
GTCTTAC13400.025.4492741
TCTCGCT18150.025.3880862
GTGTTAG22450.024.8860931
GTGTTAC13850.023.574641
GTGTAAA17800.023.2347071
GTGTATA18500.021.5711521
CGCTCTG19700.021.5611065
GTATAAG9500.021.385371
TTGCTCT42700.021.2435914
GTATTAA9700.020.9444351
GTGCTAC11550.020.7307151
GGTATAC6300.020.7307151
Produced by FastQC (version 0.11.5)