FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150866
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14100.036.978951
TCTGTCG16600.035.9882938
TCGCTCT16750.034.808244
GTCTTGC30250.032.0452651
GTCTTAC12400.028.4272521
CTGTCGC23100.027.419659
CTCGCTC19350.027.1552493
TCTCGCT20200.026.0125772
GTCTCAC40100.025.6388781
CGCTCTG20550.025.5711635
GGTATAC7350.024.9785961
GTGTTAG24550.023.9306141
GTATAAT10650.022.7551481
GTATAAC5500.022.698731
GTATTAG9200.022.3504121
GTATCGA1650.00241508722.2536581
GTATTAA10100.021.812991
TTAGCGC2654.0279083E-621.7298133
TTGCTCT40150.021.1547724
GTATAGG9150.020.8673631
Produced by FastQC (version 0.11.5)