FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109410
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.038.6917278
CTGTCGC14750.034.653779
GTCTCGC14400.032.7640231
GTCTTGC29900.028.8883111
TCGCTCT14400.027.4969394
CGTTTCG1856.1483515E-627.240219
GTGTTAT12750.026.18751
GTATAAT9150.025.3848881
GTATTAG10000.024.6788711
TCTCGCT16650.024.6463012
GTATAGG8100.024.1949711
GTATATA12500.023.8078521
GTGTACG4002.7284841E-1123.5900971
GTCTCAC38200.022.9915561
GTATCGC1902.1772823E-422.9215531
CGCTCTG16700.022.4166665
GTGTTAG23200.021.9006211
CTCGCTC17650.021.617993
GTATTAA9750.021.589361
GGTATAC6150.021.2443661
Produced by FastQC (version 0.11.5)