FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268296
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.040.1966638
CTGTCGC16500.031.8519049
GTCTCGC15400.031.560121
GTATTAG12250.027.832121
GTATAGG9550.026.5857681
GTCTTGC30700.026.2282411
TCGCTCT17100.025.6820814
TCTCGCT18300.025.1801662
GTGTTAG23000.024.9163151
GTACTAA7050.024.6948641
GTATCGG1500.001490060824.180391
CTCGCTC20000.022.6791573
GTATTAT9950.022.6007651
GTATATA14600.022.358581
GTATAAC5550.022.2198181
GTTAGCC18850.022.1530253
CGCTCTG19250.022.0657025
GTGTTAT14850.021.9821721
GTATTGG9900.021.9821721
GTATTAC8250.021.9821721
Produced by FastQC (version 0.11.5)