FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3675543
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15250.041.543998
GTCTCGC17350.039.7348561
CTGTCGC18600.034.835739
TCGCTCT20050.033.3936544
TCTCGCT21250.031.8466872
GTCTTGC34500.029.6583921
CTCGCTC23250.029.416443
CGCTCTG21300.029.4059375
GTATTAG12500.027.2857191
GTGTAGG22050.025.3413451
GTCTTAC14150.024.6168421
GTATAAT11800.023.9844681
GTGTAAG20000.023.9475731
GTGTAGT16450.023.8218441
GTATAGG8800.023.0899471
GTATAAG10600.022.5920471
GTATAGC8450.022.3287351
GTTTCAC55150.022.322363
GTGTAAC10750.022.276811
GTGTATA18700.022.119781
Produced by FastQC (version 0.11.5)