FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3675543
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18450.039.005581
TCGCTCT20000.032.7532124
GTCTTGC35950.029.8229771
TCTGTCG19450.029.605448
TCTCGCT23750.029.400222
CTCGCTC22950.028.2294433
GTATTAA11600.027.2212141
CTGTCGC23800.026.9165929
GTATTAT12950.026.651711
GTCTTAC14400.026.5178241
TAGTACG1658.843024E-526.1763924
CGCTCTG23650.024.6558515
GTGTTAC15250.024.5582471
GTATTCG2401.4313046E-624.4779931
GTGTTAG28300.023.353561
GTATTAG10900.022.906011
GTCTCAC42900.021.568021
GGTATAC7500.021.5406341
GTCCTAC12350.021.4058531
TTGCTCT50650.021.3183844
Produced by FastQC (version 0.11.5)