FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370796
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12450.038.7428748
GTCTCGC15000.035.7896351
CTGTCGC17150.034.4229
TCGCTCT17750.030.0137124
GTCTTGC32450.029.9575921
TCTCGCT18100.028.639542
GTCTTAC13850.027.2376961
CTCGCTC19350.026.4169733
TTCACCG10650.026.3633985
GTCTCAC40800.024.5378061
GTATTAA10950.024.5134491
GTATTAC8650.024.3219681
GTGTTAC13250.024.090931
ACCGTGT12400.023.8039238
CGCTCTG19100.023.746175
GTATTGG11550.022.6119141
GTATAGG8000.021.7639661
GTATAAT10350.021.7289181
GTGTTAG22750.021.6842441
GTTTCAC55700.021.5857413
Produced by FastQC (version 0.11.5)