FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652824
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16650.043.136551
TCGCTCT18950.040.649684
TCTGTCG14950.039.4871038
CTCGCTC20700.034.7790873
TCTCGCT20950.033.6781652
CGCTCTG21050.031.8063185
CTGTCGC19150.031.2026829
GTCTTGC33050.030.7313061
GTCTTAC13800.026.8111551
GTATTAA11350.025.5675181
GTATAAT11500.024.6031781
GTATTAT11650.024.2863981
GTGTTAG25450.022.8048171
GTATTAC10550.022.6926861
GTACAGA20050.022.4337431
GTCCTAC12400.022.2323991
GGGTTTC66200.021.5890061
GTGTATA20250.021.4956511
GTGTAAA22350.021.4235211
GTGTAAG18300.021.4075571
Produced by FastQC (version 0.11.5)