FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652824
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16550.036.3591231
TCGCTCT18050.032.3033184
TCTGTCG18950.030.0044658
GTATTAG11950.029.476161
CGCTCTG19600.028.2804225
GTCTTGC35450.027.9457071
CTCGCTC20500.027.740393
GTACTAG7700.026.6848551
TCTCGCT21500.025.4457052
GTGTTAG24150.024.005321
GTCTCAC41500.023.8716951
GTATATA18100.023.5151061
GGTATAC7550.023.327161
GTATTAT13000.022.5794931
GTGTACG4255.2750693E-1122.4466721
CTGTCGC26950.022.433069
CTGTTGC37800.022.2772759
GTATAAC7250.022.2680511
GTGTAAT15200.022.2081181
GTATAGT10300.022.086251
Produced by FastQC (version 0.11.5)