FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289836
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13600.035.46718
GTATACG1751.0047188E-733.164081
GTCTCGC14300.032.468331
TCGCTCT16350.030.8226534
CTGTCGC16400.030.2897019
TCTCGCT16400.029.4135272
GTCTTGC32150.029.3344841
GTATAGA9650.028.5675051
CTCGCTC16900.028.1160033
GTATTAG9950.027.7061751
GTGTAGG16750.025.986781
CGCTCTG19300.023.8732995
GTATTAT9150.023.7857151
GTATAGC6750.023.6447621
CTATACT8350.023.2791444
GTGTTAG23900.023.0691571
GTGTTAC12850.022.5825461
GTCTCAC40700.022.4590911
GTATATA12650.022.366091
ACTGTCG3305.0604285E-821.8160368
Produced by FastQC (version 0.11.5)