FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289836
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC31200.036.9271661
TCTGTCG17450.033.8215148
GTCTCGC15150.032.9381
TCGCTCT16600.030.352724
GTCTTAC12400.029.5902921
TAATACT9800.028.6448234
GTATAAT9900.027.4263131
GTACGGA2755.9553713E-926.6850621
GTGTAAT11700.025.7157331
CTCGCTC18300.025.5660293
GTGTAAC10900.024.910141
GTATAGC7150.024.6323661
CGCTCTG19300.023.1235875
CTGTCGC25250.023.0889879
GTATAGG8100.022.6493591
TCTCGCT20250.022.3932192
GTGTTAC13950.022.0940841
GTATAGA10000.022.0151751
CTCGATC12450.021.9679168
GTATTAA10700.021.9465941
Produced by FastQC (version 0.11.5)