FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258391
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15250.037.5904661
TCGCTCT16300.031.7978784
TCTGTCG16600.031.219868
CGCTCTG17650.028.5509075
GTCTTGC30950.028.495321
TCTCGCT19050.028.3417172
GTACTAG7750.026.5527341
CTCGCTC19300.026.1096233
GTGTTAC13800.026.0957581
TACACCG2508.398274E-825.9160655
CTGTCGC24350.023.0569699
GTCTCAC38950.023.0199991
GTCTTAC13150.022.9145341
GTATACG1952.3861126E-422.6135941
TAGCCCG3605.2714313E-921.996665
TATACTT10850.021.8952925
GTATATA13800.021.8352281
GTATTGA10900.021.5762731
TTGCTCT42750.021.553964
GTATTAA9750.021.106021
Produced by FastQC (version 0.11.5)