FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983359
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.038.8360188
GTCTCGC14150.033.0756261
TCGCTCT15850.032.2348334
CTGTCGC18300.028.7056989
TCTCGCT17450.026.4022562
GTATAAC5850.025.8472651
GTATTAA8650.025.8045751
CTCGCTC18150.025.7806113
GTATTAG13150.025.1873931
CGCTCTG18850.024.8140895
GTGTAAG15700.024.7653941
GTCTCAC34950.023.6920051
GTATTAC8250.023.5646481
TACACTG16800.023.1302785
GTCTTGC26650.022.965341
GTCTTAC12000.022.800981
TTACACT14250.021.7145044
GTATATA14750.021.4788881
GTGTTAC13200.021.2736421
GTGTTAG23750.020.6156231
Produced by FastQC (version 0.11.5)