FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384864
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9500.036.3594258
TCGCTCT8900.032.3421864
GTCTCGC9800.032.3254471
GTCTTGC22550.027.138781
GTCTTAC9700.025.2362671
GTTAGCC12100.024.9851343
GTATTAG7550.024.7938691
CGCTCTG11350.024.0927935
CTGTCGC13800.023.987129
GTGTTAG18200.023.7354681
GGTATAC4650.023.2250291
TCTCGCT12500.023.0339132
TTGCTCT28800.022.9876584
GTATTAA7200.021.9992641
CTCGCTC12500.021.8822173
TGTTAGC15300.021.641342
TAACACG4701.0913936E-1121.4352974
GTCCTAC7400.021.4046881
GTCTCAC28600.021.3978861
GTATAAG6800.021.175761
Produced by FastQC (version 0.11.5)