FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384864
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11750.034.9276241
GTCTTGC21500.034.1581041
TCTGTCG13350.033.4381688
GTATAAC3900.033.2307361
GGTATAC4350.031.4482441
TCGCTCT13100.030.2289774
CTGTCGC16050.028.710259
GTCTCAC27200.027.5293831
GTCTTAC8400.025.714261
TCTCGCT14650.025.0648212
GTATTAG7700.024.3116631
TCTGTCA30600.023.9999758
CGCTCTG15200.023.6841855
CTCGCTC14750.023.4304833
GTATTAC5350.022.878481
GTGTTAC8850.022.7796381
TTGCTCT29450.022.7368184
GGAGTGC56150.022.6963275
GTTTCAC32200.022.5838283
GAGTGCA63600.022.4150736
Produced by FastQC (version 0.11.5)