FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796166
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10700.035.6618041
TCTGTCG12700.033.997058
TCGCTCT12950.030.5623444
TCTCGCT13500.029.3261262
CTGTCGC16050.028.6945029
CTCGCTC14700.026.9321543
GTCTTAC12350.026.81651
GTATAGG8250.023.562481
GTGTAAA17300.023.305221
TTACACT14000.023.1301384
GTCTTGC25950.022.750331
GTGTAAG17150.022.249641
GTGTAGA18150.022.2137891
TAGTCCG1650.002717335221.806195
GTATTAG11300.021.6626571
GTATAAG9650.021.6362481
GTATAGT9000.021.5989441
GTATTAC7050.021.4457591
CGCTCTG16950.021.2272665
GGTATAC6200.020.9022031
Produced by FastQC (version 0.11.5)