FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216346
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14300.038.2602921
TCTGTCG12450.034.6798368
TCGCTCT15450.033.535074
CGCTCTG16350.029.9285935
CTCGCTC16700.029.7401083
TCTCGCT17250.029.2091482
GTCTTGC30250.028.7959061
CTGTCGC16300.027.371549
GTATTAC7900.024.6040941
GGTATAC7450.024.1576371
GTATAAT11000.022.9058321
GTATTAG13950.022.7064481
GTCTTAC14450.022.4189551
GTATAGT11650.021.6278251
GTCCTAC10350.021.5621511
GCGTAGT5051.8189894E-1221.3830971
GGAGTGC71650.021.1914865
GAGTGCA80650.021.1465326
GGGTAGA19750.020.4122851
GTCTCAC39200.020.3848551
Produced by FastQC (version 0.11.5)