FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216346
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.034.1052068
GTCTCGC13750.031.9427551
GTCTTGC28500.027.5376511
TCGCTCT15550.027.3182814
GTATTAG12300.026.927621
CTGTCGC17200.026.372059
TCTCGCT16650.025.513472
CTCGCTC16600.024.7228473
GGTATAC6750.023.4673561
GCGTAGT3702.8376235E-1023.3520361
GTGTTAG24950.021.643921
GTCTTAC13000.021.6006341
GTGTTAC13700.021.5480771
GTCTCAC35950.021.4303931
GGAGTGC69900.021.4248565
GAGTGCA78650.021.3299066
CGCTCTG20500.021.0731355
GTATAGC9300.020.903841
GTATAAG10050.020.776731
TTCACCG10400.020.7691965
Produced by FastQC (version 0.11.5)