FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636710
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC22750.034.8090131
TCTGTCG11200.033.410628
GTATAAC4950.030.5417921
CGATCCG950.004904339630.2995075
GTCTCGC12850.030.253181
GTCTTAC10250.029.4989011
TCGCTCT13200.029.4387284
CGCTCTG14800.025.7699365
GTACTAC4200.025.7112031
TCTCGCT15350.025.321152
GTCCTAC8850.025.2173141
GTCTCAC32450.025.0694121
CTCGCTC15600.024.9153613
GTGTTAC11900.024.8037471
CTGTCGC15450.024.2199979
GTTTCAC42000.023.3070683
GGGTTTC47750.022.9166241
CGGTACT2252.8110186E-522.3879724
GTGTAAT11750.022.0569291
GTATAGG8850.021.9634671
Produced by FastQC (version 0.11.5)