FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636710
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12550.036.1434258
GTCTCGC11600.034.1385731
GTCTTGC24850.033.610291
CTGTCGC17250.027.9652129
TCGCTCT14100.027.0638264
GTATAAC4550.026.9016061
GTCTCAC31950.026.8174061
GTACTAC3800.026.5268151
GCGTAGT3306.730261E-1126.1823141
GTGTTAG17600.026.1823121
TCTCGCT15550.025.4662342
TTGCTCT32150.025.0824224
CTCGCTC15350.024.8599323
GTAATAC6400.024.7499963
GGGTTTC46400.024.0521771
GTACTAT6050.023.80211
GTTTCAC40000.023.3999983
GTTAGCC14450.022.9204123
TCTGTCA35550.022.8860728
CTTGCTC32850.022.7945183
Produced by FastQC (version 0.11.5)