FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519693
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11000.032.055368
GTCTCGC11350.031.0822871
GTACGGA1952.7741407E-729.5371191
TCGCTCT11550.027.4137174
GTCTTAC9500.027.2829691
GTATTAG9100.026.1087021
GTCTTGC23850.025.9610821
GTATAGC4850.025.2359661
GTATTAA8300.025.1554811
TCTCGCT14100.023.9950982
CTGTCGC15200.023.1979569
TAATACT7500.023.0275234
GTGTAAG13950.022.7086431
GTCCGGA3203.65053E-822.4989781
GTATAAT8150.022.0848831
GTGTAAC8300.021.685761
CTCGCTC15300.021.6426373
GGGTTTC43750.021.5578771
CGCTCTG14800.020.907595
GGAGTGC56250.020.8527015
Produced by FastQC (version 0.11.5)