FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629790
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.043.072168
GTCTCGC11150.040.6830751
GTCTTGC24550.034.0215871
TCGCTCT12250.032.326464
CTGTCGC16400.031.1712599
CTCGCTC15100.027.6555713
GTCTCAC29400.027.4295581
TCTCGCT14700.027.4285132
CGCTCTG14250.027.2841535
GTATTAG9000.026.400951
GTATTAC6450.025.675341
CCGTAGA2001.1249718E-525.2004269
CTTGCTC32800.025.0243363
GGCTAGA16550.024.7984751
TAATACT8450.024.7100054
GTACTAT7400.024.32521
TTGCTCT34900.024.1374864
TTACTCT14200.023.8309364
TACCCCG3052.1931555E-823.6065085
GTGTAAA17050.023.2266391
Produced by FastQC (version 0.11.5)