FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445993
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.046.0273978
GTCTCGC13650.040.6153831
TCGCTCT13050.035.3103454
CTGTCGC15400.035.0649389
TAATACG1459.18948E-734.758624
GTCTTGC21350.034.060891
TCTCGCT14850.033.4545442
CTCGCTC15600.029.0769213
CGCTCTG15950.027.536055
GTCTTAC9750.027.3230781
GTACTAG4350.026.4827581
TGTACCG1959.2446735E-625.8461535
GTGTTAC9750.025.1076931
TTGCTCT29650.024.7689724
GTGTTAG15050.024.3986721
GGCTAGA14600.023.6712341
CTATACT6100.023.6065584
CTTGCTC28900.023.169553
GTCTCAC28350.022.8571411
GTTTCAC34200.022.7368413
Produced by FastQC (version 0.11.5)