FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153781
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.038.3477068
GTCTCGC8700.033.106421
CTGTCGC10750.031.4789739
TCGCTCT9700.028.2060994
GTCTTGC18750.028.0345171
GTATAAC4200.027.4310361
CGCTCGT1050.00797531627.4284887
GTGTAGT10950.026.3037321
GTATTGC6400.025.8773231
GTATTAT5350.025.5723881
GTATTAC5000.024.4821971
TCTCGCT11200.024.4307652
GTTTCAC29450.024.2059083
TACACTG11700.023.9999265
GGGTAAC6700.023.6439131
GTGTAGG11950.023.5000171
GTATTAA5850.023.3867151
GTGTTAC8650.023.3084531
GGAGTGC48400.023.0577815
CTCGCTC12200.023.018463
Produced by FastQC (version 0.11.5)