FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095513
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.040.5514768
GTCTCGC9200.039.130191
GTTACGC1201.0034162E-535.999783
GTCTTGC19000.033.726111
CGTTTCG1153.294402E-431.3041559
TCGCTCT9550.030.1568854
CTGTCGC12050.028.680329
TCTCGCT12050.027.4853082
GTGTTAT7650.027.2939531
CGCTCTG10650.027.042095
GTATTAG6800.026.4704271
CTCGCTC11900.024.806573
GTGTTAG12400.024.3869481
TTGCTCT26750.024.224154
TACTTCG1500.001556339423.9998517
GTCTAGC9800.023.510061
TGTTACG1550.001883528223.2256642
GGGTTAC6550.023.0838281
GTGTAAC6400.022.4998631
CTTGCTC25600.022.2186153
Produced by FastQC (version 0.11.5)