FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881804
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7550.043.8674778
TCGCTCT8000.040.499934
GTCTCGC8400.039.431651
CTGTCGC9550.033.9266479
TCTCGCT9700.031.9200172
GTATTAT6100.031.871341
CGCTCTG9100.031.64835
CTCGCGA950.0048911630.315749
CTCGCTC10050.030.09193
TGTAGCG1358.418948E-426.6666225
GTGTAAG9600.026.2520481
GTCTTGC16000.025.6520021
TTATACT6500.025.4768814
GTGTTAC9100.025.3206581
GTGTAAC5250.024.6876411
TAATACT6550.024.1831654
GTCTCAC21000.024.0018731
GTACTAT3952.5465852E-1123.6980511
GTGTATA9000.023.2018131
GTACTAC2852.97121E-722.7386171
Produced by FastQC (version 0.11.5)