FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881804
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.036.6242338
GTCTTGC16000.032.3999561
GTCCTAC6600.028.36361
CTGTCGC12200.027.737679
GTCTCGC6650.027.0676351
GTATAGC3751.2732926E-1124.9599671
TATACTG6100.024.7868545
GTGTTAG11950.024.7028981
GTCTCAC21700.024.5529651
GTACTAA3251.6116246E-924.36921
GTATTAG6100.023.6065271
GTACCGA1550.00188340423.2257771
GGATCGA1550.00188340423.2257771
ACCGTGT5800.022.3447998
ATACTAT6250.021.887976
CTTGCTC21750.021.8482483
GTATTAA6600.021.8181531
TTGCTCT23200.021.724114
GTCTTAC7350.021.5509911
GGCGTCG1700.0032205121.1764431
Produced by FastQC (version 0.11.5)