FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966788
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7550.042.9136858
GTCTCGC9150.041.7089461
CGTTTCG1054.012536E-641.1426479
CTGTCGC9450.038.0950439
TCGCTCT9750.036.184434
TACCCGT1751.07338565E-732.9141165
TAGCTCG1352.2471728E-531.9998349
TCTCGCT11200.031.5030422
GTCGTAG1402.8791428E-530.8601231
CTCGCTC11800.029.9011943
CGCTCTG10850.029.198015
TGTACGC1504.6104593E-528.8027822
GTATTAC4050.028.4471931
GTCTTGC16150.026.751811
ATACCCG1658.828831E-526.1816834
GTCCTAA7350.025.471851
GTATAAG4950.024.7296621
GTATAAC3501.4188117E-1024.6880991
TATACTG4750.024.2525065
GTGTTAG11300.024.2147271
Produced by FastQC (version 0.11.5)