FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966788
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9200.039.129911
TCTGTCG8250.038.3994838
TCGCTCT10100.034.2173654
CGCTCTG9650.029.8441585
CTGTCGC11800.029.287749
CATACCG1306.753489E-427.6919365
GTCTTGC15900.027.1694451
GTATTAG6100.027.1471771
GTATTAC3800.026.5259591
GCACGAC1907.5562166E-626.5259593
ATACCGT1658.829328E-526.1814676
GTATTGA4400.026.1814651
GTCTCAC20650.025.8011071
GTGTATA9000.025.5996551
TCTCGCT12950.025.0189692
GTCTTAC8600.024.2787441
CTCGCTC13100.024.1828823
GTGTTAG11850.023.6958851
GTGTAAG9250.023.351041
ATGGCGT8350.023.2811246
Produced by FastQC (version 0.11.5)