FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141374
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8300.035.5676881
TCTGTCG7650.034.82338
GTATTCG1102.537514E-432.7285841
CTGTCGC10200.028.9409839
GTATTAT6400.027.0010811
GTATAGC4600.026.6097621
CTTGTCG1907.5562275E-626.5261389
GTATAAC4150.026.0251371
TCTCGCT10350.025.7401622
GTCTTGC19000.025.0115281
GGTATAC3851.8189894E-1124.3126621
TCGCTCT9900.023.9998424
GTAGTAC3352.2882887E-923.6427363
GTGTAAT8600.023.4427991
GTATTAA6150.023.4155731
CTCGCTC11150.023.2475683
GTGTAAC6900.022.957441
GTATAAT5650.022.938971
GTCCTAC6600.022.910011
ATCCGGT1902.2989463E-422.7377531
Produced by FastQC (version 0.11.5)