FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171662
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC1002.867302E-643.203751
TCTGTCG8150.042.4046828
GTCTCGC9100.035.6074871
CTGTCGC10750.035.4974869
GTCTTGC17550.034.464531
TCGCTCT9550.031.6647554
CGCTCTG10750.028.1300835
GTGTTAC7550.026.7043061
TCTCGCT11100.026.5969032
TATACGT1658.823727E-526.184092
GTGTTAG13100.025.8343031
GTATAAC3109.313226E-1025.5506081
CTCGCTC11300.025.488943
TTACACT8300.024.2890284
GTCTCAC21550.024.0577741
GTATTAT5150.023.7690561
GTGTAAA12550.023.5239541
GTCTTAC7750.023.2278251
GTATTAG5000.023.0421
GGAGTGC46300.022.859495
Produced by FastQC (version 0.11.5)