FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171662
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.041.3537568
GTCTCGC8550.037.052551
GTCTTGC18600.033.6773451
TCGCTCT10150.031.2117524
GGTATAC2752.3101165E-1028.7999361
CTGTCGC15200.027.4736219
TCTCGCT12100.026.77682
CGCTCTG11450.026.4104215
GTACGGA1959.2442115E-625.8460961
ATACTGA10050.025.7909876
TAATACT6500.025.4768664
GTGTAAC5550.024.6485941
ATAATAC5850.024.6153283
GTCTCAC21750.024.4964981
GTACTAT3851.8189894E-1124.3116341
TTGCTCT25400.023.810974
CTCGCTC12100.023.80163
GTATTGG5500.023.5635851
GTATAAG5600.023.1428051
GGGTTTC31700.022.9400121
Produced by FastQC (version 0.11.5)