FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820975
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6750.047.999788
CTGTCGC8900.038.8312849
GTCTCGC7800.036.0008241
TCGCTCT8150.033.5703964
GTCTTGC14850.033.455311
TTGCGCA1102.5380307E-432.7271234
TACATCG1153.2942038E-431.3042055
TAGCCGT1204.227405E-429.9998636
GTATTAA5800.029.7937851
TTCCGTA1703.1798882E-629.6477382
GTACGGT1000.006283960328.800661
GTATATA7150.028.196451
CGCTCTG8850.027.6608895
TACGTCC1050.00797505627.4284468
GATCCGT1856.1414194E-627.243126
GTACTAA3453.6379788E-1227.1310541
GGGTACG1607.159544E-527.0006181
TCTCGCT9100.026.9017142
CTCGCTC9450.026.6672763
TAGCGGT1358.418988E-426.6665444
Produced by FastQC (version 0.11.5)