FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239924
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.045.85468
CTGTCGC10800.039.999879
GTCTCGC9700.034.890381
TCGCTCT10100.033.504844
CGCTCTG10950.030.2464755
TCTCGCT10800.029.3365142
GTATAGG5700.029.055781
GTATTCG1753.983103E-628.8031221
GTCTTGC21050.028.7347031
GTATTAT7450.028.0298841
GTATTAA6100.027.1504821
CTCGCTC12200.027.1498763
GTGTAAT9900.026.9120081
GTATTAC5150.026.5659851
GTATAGT5350.025.5728651
GTGTTAG13600.024.3555811
GTATAAC3602.0190782E-1024.0026021
GTACTAC4500.024.00261
GTATTAG7950.023.549721
GTGTACG2151.9681129E-523.4444031
Produced by FastQC (version 0.11.5)