FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239924
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.036.177251
TCTGTCG10250.032.3121158
GTCTTGC20300.032.275781
TCGCTCT11300.031.8583284
CTGTCGC13100.028.0304649
GTCTTAC9250.028.0215511
TCTCGCT12700.027.7794592
CTCGCTC12600.027.4285053
CGCTCTG12650.026.7509215
GTATTAC6100.025.9671481
GTATAAT5950.025.4117031
GTGTAAA13900.024.3452631
CTATGCG2101.6418762E-523.9999439
GTATTAG7300.023.6711731
GTGTAAG11300.023.5751631
GTGTAAT8900.023.4606151
GTATAGA6150.023.4145761
TTGCTCT27950.022.9265984
TCCGTAC2252.7991928E-522.3999443
GTGTATA11100.022.0539991
Produced by FastQC (version 0.11.5)