FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341533
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.036.924321
TCTGTCG10650.032.4498068
GTCTTGC19550.032.4422571
TCGCTCT11800.029.906424
TCTCGCT12300.029.8617612
GTATTAA7600.028.4500371
GTACTAT5200.026.3345221
GTCTTAC8550.026.1318871
GTTAGCC13050.025.9380743
GTGTTAG16450.025.8500461
CTCGCTC13150.025.7408263
GTGTTAA10100.025.6895391
CTGTCGC15100.024.793499
GTATTAG8200.024.610441
GTATACG2401.6838985E-624.0244771
ACCGTGT7050.023.4887148
GTCTCAC26450.023.4340821
TACGCTC1550.001883828523.2251665
GAATACG1902.2838432E-422.7600291
CGCTCTG13650.022.6806935
Produced by FastQC (version 0.11.5)