FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341533
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.033.137218
GTCTCGC11100.032.4606971
GTCTTGC21900.030.601991
GTATAAG5950.026.645051
TCGCTCT12950.026.130594
ACCGTGT7850.025.680868
GTATAAC5150.025.1869811
CGTTTCG1450.001277631624.8269379
GCGTTAG1751.3091016E-424.707231
GTGTTAC10850.024.5743961
GTATACG2351.4037469E-624.5320011
GTACTAA6200.024.4083521
CTGTCGC17300.024.13819
CTTAACG1500.00155657523.9993722
GTACTAG3952.5465852E-1123.7168561
GTAACAC9500.023.4941233
TCTCGCT14600.022.6843382
TTGCTCT32500.022.5963344
GTGTAAT9700.022.2874491
GGAGTGC52600.021.763695
Produced by FastQC (version 0.11.5)