FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108764
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.034.2480668
TCGCTCT11650.032.7695734
GTCTCGC10950.029.6180341
GTATACG1804.925676E-628.0274371
GTGTAAC5200.026.333471
GTACTAC3306.730261E-1126.2074721
CTCGCTC11200.025.725443
GTATAAT6850.025.2509981
TCTCGCT12700.024.9557022
CGCTCTG12950.024.4629045
GTCTTGC18150.024.222061
GTATTAG7200.024.0235181
CTGTCGC13800.023.9990239
CTATCGC2151.970312E-523.4409079
TCTATCG1550.001883650923.225418
GTCGAAG2202.3351413E-522.9315381
TTACACT9850.021.9384464
GTACTAA4659.094947E-1221.698661
GTCTAGC8800.021.2935711
GAATACG1700.003202372521.1972221
Produced by FastQC (version 0.11.5)