FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108764
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8250.041.0473631
TCGCTCT9950.034.7328344
GTACTAT3400.033.906461
TCTGTCG9850.032.8926128
TAGTCCG950.00489182830.315065
GTATTAA5250.030.1928961
CGCTCTG11350.028.545575
TCTCGCT11850.026.7335362
CTCGCTC12250.025.8606033
GTATTAG7400.025.3152961
GCGTAGT2351.4055913E-624.528081
GTGTAAC6250.024.2092131
GTGTTAG14950.024.09741
GTCTTGC16700.023.7294461
GTATAGG4950.023.2892881
GTATAAG5650.022.9543741
TAATACT6300.022.8565944
TAACACG5050.022.8113334
CTGTCGC14650.022.6069649
GTCTCAC24400.022.4421881
Produced by FastQC (version 0.11.5)