FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347648
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9500.034.104098
GTCTCGC10050.033.693451
TCGCTCT11300.030.591494
GTCTTGC22050.029.733531
TCTCGCT13350.028.0517482
GTATATA12250.027.6423821
CTCGCTC12700.027.2192023
GTATTGC7200.026.016841
CGCTCTG11850.025.518115
GTATTAA8850.023.6085011
GTATTAG9500.023.5099541
GTCTTAC10200.023.3092041
CTGTCGC13350.023.1902189
GTATTAC6000.022.8147681
GGAGTGC53050.022.39325
ACCGTGT8400.022.284958
TACGTAC1650.00270473921.8234752
GAGTGCA57900.021.7609126
GTGTAAC5700.021.4875931
GTGTAGA13150.021.3674431
Produced by FastQC (version 0.11.5)