FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645218
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT12000.030.5995854
TCTGTCG11950.030.1251168
GTCTCGC11500.029.4457261
GTACTAT5550.027.2614251
GTGCTAC8400.026.5891631
GTCTTAC9350.025.4287241
TACACCG1701.0817037E-425.4114235
GTCTCAC29550.025.11321
GTACTAG6100.024.8034291
GTCTTGC24050.024.2656651
CGCTCTG14250.024.2523025
GTGTAAA15700.023.8630491
GTGTTAA10600.023.7894521
CTCGCTC14650.023.5901243
ATAATAC8750.022.2168433
GGTATAC5550.022.0687731
ACCATGT32750.021.5447468
GTACAAG9700.021.540141
GTTTCAC38000.021.2207663
TCTATCG1700.003221078321.1761868
Produced by FastQC (version 0.11.5)